Proteomic profiling analysis of rapeseed under salinity stress

Rapeseed as one of the crops which is being cultivated in order to produce edible oil, have essential importance, however, it is severely influenced by salt stress. In the aim of studying effects of salinity on the growth characteristics and proteome profiling changes, Sarigol (salt-sensitive) was cultured under greenhouse conditions and salinity stress exerted at three levels, including 0, 175 and 350 mM NaCl. Seeds were sown in the current hydroponic system. To study the changes of proteome profile, 2-dimentional gel electrophoresis technique was used. The results indicated that fresh weight, shoot dry weight, shoot height, grain yield, and 100-grain weight significantly decreased with increasing intensity of salinity stress and proline concentration increased in leaves as well. Comparing the proteome profile of seed demonstrated that 29 spots with differentially expression changes occurred under 350 mM NaCl. Differentially expression spots were identified and grouped in six functional categorize, including carbon metabolism, defense/detoxification, storage proteins, photosynthetic, nitrogen metabolism, and synthesis/degradation. The majority of identified proteins involved in carbon metabolism and defense/detoxification. Salt stress decreased significantly the growth characters of Sarigol and altered proteome profile and protein expression of seeds. More than 60% of spots indicated significant down-regulation.