Comparison of different DNA extraction and preparation methods for forensic application of Blood Samples

Extraction of DNA from forensic samples is the first and one of the most crucial steps of any genetic forensic test. Hence, obtaining a suitable protocol of extraction DNA that is easy to extract, cost, time, and quality which is essential in the forensic genetic research. The present study was carried out to achieve a suitable, faster and low cost method of DNA extraction from different sample for further molecular study. After preparation of samples, three different DNA extraction methods, including chelex, non-enzymatic and modified rapid alkaline lysis were evaluated. In order to compare the above mentioned methods for evaluation ease of extraction, low cost and time , the quality and quantity of DNA obtained from each method considering OD, DNA concentration and PCR efficiency were investigated. The findings of this study show that despite the availability and lower cost and time of manual methods, these methods can be substitute for kit method.