Antibacterial properties of a bacteriophage cocktail against Salmonella enterica serotypes (enteritidis, typhimurium, infantis) compared to ciprofloxacin in vitro: First Report From Iran

Salmonellosis is a gastroenteritis caused by the infection with different serotypes of Salmonella. Salmonella enterica is an enterica subtype, enteritidis, typhimurium, and infantis are the most important factors of gastroenteritis in humans. In recent years, increasingly commonly used antibiotics and treatments are resistant. The main purpose of this study was to compare the antibacterial effects of lytic bacteriophage Cocktail and ciprofloxacin on Salmonella enterica strains in laboratory conditions. Standard strains of salmonella (enteritidis, typhimurium, and infantis) were collected from the Faculty of Veterinary Medicine, University of Tehran, and their specific bacteriophages were isolated by soft agar method. Specificity of bacteriophages was investigated for strains of Salmonella and Staphylococcus aureus, Shigella dysenteriae and Enteropathogenic E. coli (EPEC) bacteria using spot test. Antibiotic sensitivity was determined using E-Test method. In order to obtain an appropriate headline for evaluating the therapeutic results, different concentrations of bacteriophage with bacteria were evaluated. Finally, the preventive and therapeutic effect was evaluated in comparison with ciprofloxacin. Different concentrations of bacteriophages have the ability to reduce and eliminate strains of salmonella (enteritidis, typhimurium, and infantis). Bacteriophage specific plaque against other strains of Salmonella and other intestinal pathogenic bacteria did not have any lytic function. The results showed that bacteriophages function as specified. Due to the increasing prevalence of antibiotic resistance and treatment concerns, bacteriophages can be a good alternative to the use of antibiotics in the treatment of bacterial infections