Detection of FGFR3 Gene S249C Mutation in Urinary Epithelial Cell by Tetra-Arms-Pcr Method in Patients With Bladder Tumors
Message:
Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
Introduction
Genetic variation of FGFR3 gene is one of the factors affecting the bladder tumor. FGFR3 is a tyrosine kinase receptor, involved in controlling the cellular and angiogenesis cycle. This protein affects a variety of diseases and cancers and cartilage growth abnormalities. Regarding the high activity of fgfr3 mutations in more than 50% of primary tumors of bladder urethral cells, it was suggested in this stage that the gene is involved in tumor-forming epithelial cells.
Objective
the aim of this study was to detect S249C mutation in FGFR3 gene through tetra primers arms PCR and to determine the association between the presence of this mutation and the early stages of bladder cancer in the north of the country.
Materials and Methods
in this study, after separation of epithelial cell in the urine of 100 patients with bladder tumor, their DNA was extracted using a commercial kit. After bioinformatics studies, primers with appropriate pair of mutants were designed and synthesized for the S249C mutation. After optimization of the reaction, screening was performed on the population and the data were analyzed using SPSS software.
Results
Our results showed that primers designed to differentiate well between normal alleles and mutants in the epithelial cell of 28 patients with bladder tumor ,S249C mutations were identified p<0.0001. Statistical analysis of the data indicates the significance of this mutation.
Conclusion
The mutation of S249C FGFR3 gene was identified in 28% of the population studied and is considered a potential risk, although more and more studies are needed to confirm the results.
Conflict of interest: non declared
Language:
Persian
Published:
Journal Of Guilan University Of Medical Sciences, Volume:27 Issue: 107, 2018
Pages:
10 to 18
magiran.com/p1894135  
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یک‌ساله به مبلغ 990,000ريال می‌توانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
  • حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران می‌شود.
  • پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانه‌های چاپی و دیجیتال را به کاربر نمی‌دهد.
دسترسی سراسری کاربران دانشگاه پیام نور!
اعضای هیئت علمی و دانشجویان دانشگاه پیام نور در سراسر کشور، در صورت ثبت نام با ایمیل دانشگاهی، تا پایان فروردین ماه 1403 به مقالات سایت دسترسی خواهند داشت!
In order to view content subscription is required

Personal subscription
Subscribe magiran.com for 50 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!