Comparison of hematological and biochemical parameters of common carp (Cyprinus carpio) fed separate and combined levels of commercial multi-enzymes

The aim of the present study is to compare hematological and biochemical parameters of common carp (Cyprinus carpio) fed with separate and combined levels of multi-enzymes commercial feed. A total number of 96 common carp fingerlings weighing 13.06±2.56 g and total length 9.25±1.34 cm were distributed in six treatments including control (without multienzyme), 1000 and 1500 mg/kg Combo, 1000 and 1500 mg/kg Natuzyme and combined treatment of 1000 mg/kg Combo plus 1000 mg/kg Natuzyme into 12 tanks at a density of 8 fish per tank. At the end of a 8-week feeding trial, blood sampling was done to measure hematological parameters. Results showed that there were significant differences in final weight, body weight increase and feed conversion ratio among treatments (p<0.05). Some treatments had an increase (p<0.05) in RBC, contents of hematocrit and hemoglobin, WBS (except 1500 mg/kg Natuzyme) and lymphocyte number compared to the control. The highest number of lymphocyte was seen in 1000 mg/kg Natuzyme and 1500 mg/kg Combo which had significant difference with other treatments (p<0.05). The maximum content of triglyceride was recorded in 1000 mg/kg Natuzyme that had significant difference with the control and combined treatment (p<0.05). There were differences between levels of liver enzymes among treatments (p<0.05). The highest content of amylase, protease and lipase were observed in 1500 mg/kg Combo and 1000 mg/kg Combo, respectively which showed significant difference with others (p<0.05). According to the results, Natuzyme and Combo can be effective in the improvement of hematological and biochemical parameters of common carp fingerlings.