Chromosomal location of HindIII Satellite DNA in Acipenser persicus and Acipenser gueldenstaedtii by using of fluorescent in situ hybridization (FISH)

In this study, a HindIII Satellite DNA (168bp) was isolated from Russian sturgeon, Acipenser gueldenstaedtii and was used as a probe for fluorescent in situ hybridization (FISH) with the chromosomes of Persian sturgeon, Acipenser persicus and Russian sturgeon. After obtaining suitable chromosomal preparations from the fishes through leukocyte culture and labeling the probe with Spectrum orange (Orange-dUTP) through Nick translation method, the probe was hybridized with chromosomes of the fishes on the microscopic slide. In the studied chromosomal preparations, the hybridization colored signals were clearly visible. Counting the produced signals in twelve chromosomal preparations of three fishes from each species showed that the mean number of the signals in the chromosomal preparations of the Persian sturgeon and Russian sturgeon were 66±4 and 68±3 respectively. In this study, accurate kind determining of chromosomes and HindIII SatDNA site on the chromosomes were impossible due to the presence of microchromosomes and heterogeneity of shapes and sizes of colored signals