Response of antioxidant enzymes, chlorophyll content and leaf area index of Rye to seed inoculation with plant growth promoting bacteria under salinity conditions

In order to study of salinity tension and seed inoculation with plant growth promoting bacteria on antioxidant enzymes activity, chlorophyll content and leaf area index of Rye, a factorial experiment was conducted based on randomized complete blocks design with three replications at research greenhouse of Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili in 2016. Factors under studied were salinity in four levels including non-application of salinity as control, salinity 25, 50 and 75 milliMolar by NaCl and seed inoculation by plant growth promoting bacteria in four levels including no inoculation as control, seed inoculation by Pseudomonas, Azosprilium and co-application Azosprilium and Pseudomonas. The results indicated that the highest antioxidant enzymes activity (such as catalase, peroxidase and polyphenol oxidase) were obtained in salinity of 75 milliMolar and co-inoculation seed with Azosprilium and Pseudomonas, and the least of them were obtained in non-application of salinity and no seed inoculation with plant growth promoting bacteria. With increasing soil salinity, chlorophyll content of a, b, total chlorophyll and leaf area index decreased and they were vice versa in seed inoculation with growth promoting bacteria. Maximum ratio of Sodium in root and shoots was obtained at the highest level of salinity and no inoculation seed with growth promoters’ bacteria and minimum in the lowest level of salinity and seed inoculation with Azosprilium and Pseudomonas. It seems that the co-application of growth stimulant bacteria can be used as a suitable means to reduce sodium in root and shoot, and increase leaf area index, chlorophyll a, b and total chlorophyll content of Rye under salinity tension.