The effect of vitamin D supplementation on the levels of Klotho in the brain tissue of female lewis rats after 6 weeks of swimming

The aim of this study was to investigate the effect of 6 weeks endurance swimming and vitamin D supplementation on Klotho levels of the brain tissue. In this study, 25 rats were divided into 5 groups: 1) healthy control 2) vehicle 3) vitamin D, 4) swimming and 5) vitamin D + swimming. Swimming training program included one hour daily for 6 weeks and 5 days per week. In addition, supplementary groups were received vitamin D supplement (2ng/0.2mg sesame oil/every other day) intraperitoneally for 2 weeks. Data were analyzed using one-way analysis of variance (ANOVA) and Tukey post hoc test. Klotho levels were measured by ELISA. There was no significant difference in Klotho levels of brain tissue between control and vitamin D groups (p=0/46).The differences between swimming (P=0.03) and vitamin D +swimming (P=0.008) was significant. The results of this study showed that swimming alone and in combination with vitamin D supplementation could increase the Klotho levels of brain tissue and consequently may have neuroprotective role against detrimental factors and leads to a significant increase in resourcesand and finally neuronal protection.