Investigation of Cytotoxic and Apoptogenic Effects of Terminalia Chebula Hydro-Alcoholic Extract on Glioblastoma Cell Line

Glioblastoma multiforme is the most prevalent group of primary brain tumors. Terminalia chebula has traditionally been used for its anti-cancer, laxative, diuretic, and anti-oxidant properties. The aim of this study was to investigate the effects of alcoholic extract of Terminalia Chebula (TCAE) on the glioblastoma multiforme (U87) cell line. Cellular cytotoxicity was measured by MTT assay and intracellular active oxygen species were measured using DCFDA (2 'and 7'-dichloro fluorescein diacetate) assay kit. In addition, apoptotic cells were detected with an Annexin V-FITC early apoptosis staining. TCAE reduced the proliferation of U87 cells in a concentration- dependent manner. IC50 was 145.3 and 192.3 μg/mL after 24 and 48 hours, respectively. Activated reactive species decreased significantly at 2 and 6 hours after treatment compared to the control group. Furthermore, induction of apoptosis occurred in the first 24 hours. TCAE caused the destruction of cancer cells enhance the free radical species of oxygen and induce apoptosis on glioblastoma cell line. It seems that TCAE has the potential to use as an adjuvant drug for treatment of glioblastoma