Spectrophotometric determination of lithium in blood serum of two species of mullet (Lizza aurata‎‏) and sardine (Sardinella Melanura) with thorin reagent in Chabahar Bay

In the present study, Thorin (Disodium 4-[2-(2-arsonophenyl)hydrazin-1-ylidene]-3-oxo-3,4-dihydronaphthalene-2,7-disulfonate) as a reagent was used by forming color in the alkaline water – acetone media for direct spectrophotometric determination of lithium in blood serum of two species of mullet (Liza auratus) and sardines (Sardinella melanora) with thorin reagent in Chabahar Bay. In December of 2016 from any fish caught 15 blood samples were taken and the serum was separated. Affecting factors such as reaction time, concentration of thorin and percent of potassium hydroxide and acetone again were studied and optimized. Under optimum conditions, the correlation coefficient, linearity range relative, standard deviation of method were obtained 0.994, 0.05-3 meq/L, 0.82% (n =7), respectively and the average concentration of lithium in blood serum of two fish species was estimated 0.22 meq/L. Also Analyzing data using SPSS was investigated. Due to the results there was no significant different in lithium concentration in blood serum of two fish species.