Investigation the antibacterial effect of silver nanoparticles made by nanophytosynthesis alone and in combination with chloramphenicol
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Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
Background and aims
Nanophytosynthesis is a part of nanobiotechnology science and it is the use of plant secondary metabolic compounds through which the synthesis of different nanoparticles is carried out. The resistance of microbes to conventional synthetic antibiotics is inevitable. To reduce this resistance, effective nanoparticles that can have an antibiotic enhancement effect have been used. The aim of this study was to investigate the effect of enhancing the antibiotic properties of chloramphenicol using nanoparticles made by nanophytosynthesis.
Methods
The cubic nanocrystallites of silver were synthesized by nanophytosynthesis method with utilization of the aerial part of Anthemis talyschensis methanolic extract. The structure and optical properties were investigated by FT-IR, UV/Vis, TEM, SEM and XRD. Antibacterial activity of synthesized silver nanocrystallites as separately and in combined with chloramphenicol against four bacterial species were studied according to the disk diffusion method.
Results
The results showed that more than 75% of the synthesized silver nano particles have a cubic structure. The biological study showed that these nanoparticles have a good activity against growth of bacteria and enhances the antibacterial effect of chloramphenicol as antibiotic.
Conclusion
Increasing the inhibition of bacterial growth by these nanocrystals was associated with the silver nano cubic structure. Also, nanophytosynthesis method for different nanoparticle preparation is an economical and green synthesis method, and it is in the adjustment of nature which it provides an optimal condition for production of nanoparticles.
Language:
Persian
Published:
Journal of Shahrekord University of Medical Sciences, Volume:20 Issue: 5, 2018
Pages:
45 to 58
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