Application of SCoT marker to discriminate Lolium perenne and Lolium multiflorum species

Lolium is an important cool season grass species growing in different climates. To identify variation among nine Lolium multiflorum and nine Lolium perenne genotypes, this experiment was carried out based on 15 SCoT molecular markers. All of the SCoT primers showed 86 visible bands. Seventy four out of 86 bands were polymorphs. Primers SC35, SC36 and SC26 (with 9 and 8 bands respectively) showed the highest number of bands and SC10 and SC44 (with 2 and 3 bands) showed the lowest number of bands. The greatest number of band for polymorphic information content (PIC) belonged to SC5، SC40، SC35 ،SC63 ،SC13, C44، SC26 ،SC21and SC10. The greatest Resolving Power (RP), Marker Index (MI), Effective Multiplex Ratio (EMR) belonged to SC35 andSC26 primers. Genetic similarity based on Jaccard coefficient ranged from 0. 35 to 0. 91. Mean similarity between the genotypes was 0. 64.  Cluster analysis grouped genotypes into two groups. The results of clustering was confirmed by principal coordinate (PCo) analysis. Analysis of molecular variance of annual and perennial species revealed that variation within species was 51% and variation between species was 49%.  Based on heterogeneity (He) and Shannon indices (I), the highest intraspecific variability and gene variation belonged to L. Multiflorum.