Genetic variability of Tanacetum polycephalum populations in West Azarbaijan using ISSR molecular markers

Tanacetum polycephalum from Anthemideae tribe of Asteraceae family is a rangeland species with high morphological diversity and vast dispersal in Northwest, East and centre parts of Iran. The purpose of this research was studying genetic diversity of 20 populations of T. polycephalum in West Azarbayjan using ISSR molecular marker. Ten ISSR primers out of 14 primers could be scored based on presence or absence of each band. Over all 208 bands were produced .The range number of bands was between 15(IS24) to 28 (IS20) and the average was 20.8. Regarding the polymorphism bands percentage, IS8, IS1, IS10, IS13, IS20 markers had high percentage (100) and IS24 marker had the lower percentage (80) and mean percentage of polymorphism of the primers was %96.03. PIC, RP, EMR and MI means were 0.30, 6.06, 19.5 and 6.13 respectively. Over all the most effective markers were IS8, IS1 and IS10. Results of cluster analysis revealed the grouping of studied populations in 2 main and 4 subgroups, in which the different populations collected from the same locality were located in the same group. Therefore, a close relationship was characterized between geographic location and genetic diversity and it may be concluded that geographic and climatic factors could be one of the responsible main factor for genetic diversity.