Comparison of ziziphus poultice, combination of olive oil and lime water and silver sulfadiazine on healing rate of third-degree burn wounds in Balb/c mice

Burns are among the most common causes of wounds. One of the medications used in these cases is silver sulfadiazine 1%, which has many side effects. Therefore, people turn to traditional compounds for treatment of these ulcers . Despite the widespread use of lime water in combination with olive oil and Ziziphus in Iran, little research has been done to prove their benefits. The purpose of this study was to investigate the topical effect of Ziziphus and olive oil and lime water combination on the healing rate of third-degree burns in mice. In this study, 24 male Balb/c mice with a mean weight of 25±5 g were used. After anesthesia, a 1 cm2 third-degree burn was created with a special device. The mice were randomly divided into four groups and received normal saline, Ziziphus poultice, olive oil-lime water, or silver sulfadiazine 1% daily. Pictures were taken weekly for three weeks. Then the area of the wounds was measured and the percentage of healing was calculated. Repeated measure ANOVA was used to analyze the data. In all weeks, the percentage of wound healing in the second to fourth groups was significantly higher than that of the first group, but was not significantly different from each other. The results of this study showed the positive effect of Ziziphus leaves and the combination of olive oil and lime water on the healing rate of third-degree burn wounds in mice.