Study of the effects of aqueous extract of Anacyclus pyrethrum on seminiferous tubules in rat testis: Short communication

Extract of Anacyclus Pyrethrum contains materials which the potential to affect the tissues of the testis. The aim of the present study was to identify the histological effects of aqueous extract of AP on Rat testis. In this experimental study 48 adult male Wister rats were chosen and divided into experimental (36 rats) and control (12 rats) groups. The rats in the experimental group were divided into three subgroups, one, two and three, and each subgroup received 50, 100 and 150 mg/kg doses of the extract intraperitoneally for four weeks, respectively. The rats were then placed under deep anesthesia and samples were collected from their testicles and paraffin blocks were prepared. Histological sections with 6 µm thickness were prepared and stained with H&E method and variables (internal and external and thickness of germinal epithelium) were measured in at least in 100 somniferous tubules in each rat. Statistical analysis showed that there was a significant difference between control and experimental groups and for external and internal diameters (p<0.002). Although there was no any significant difference for an epithelial thickness between control and experimental 50 mg/kg, there was a significant difference between control and other experimental groups (p<0.002). It seems that aqueous extract of Anacyclus pyrethrum could change epithelial thickness, internal and external diameters of seminiferous tubule Rat testis.