Determination of Optimum Conditions for Production of Hydrolyzed Protein with Antioxidant Capability and Decrease of Nitric Oxide from Tomato Wastes by Alcalas

Tomato waste can be used as a new protein source for the production of hydrolyzed protein. Free radicals are among the most important factors in the development of cancer and genetic mutations, which have become one of the greatest threats for human health in recent centuries. In this research, hydrolysis condition of tomato seed protein (temperature, time and amount of alcalase enzyme) to achieve maximum antioxidant activity and nitric oxide reducing power were investigated. The values were evaluated using Design Expert software and analyzed by the response surface methodology. Results showed that with the optimimizing hydrolysis conditions using the alcalase enzyme, it is possible to achieve a products with significant DPPH radical scavenging activity and nitric oxide reducing power. The results indicate that optimum conditions for achieving the maximum DPPH free radical inhibition activity and nitric oxide reducing power is temperature of 50 ° C, hydrolysis time 210 min, and the amount of enzyme 1.85%. Under these condition the amount of DPPH free radical inhibition activity and nitric oxide reducing power was 85.53 and 61.17, respectively. The results showed that the hydrolyzed protein produced from tomato seed could be used as a natural additive in the foods formulation and pharmaceutical uses with high antioxidants and nitric oxide reducing activity.