The Effects of Singular or Combined Administration of Pediococcu sacidilactici and Raffinoson Mucosal Immune Parameters and Intestinal Histomorphology of Gold Fish (Carassiusa uratus)

Inroduction & Objective: Mucos and its compounds as the first line to defense against pathogens is one of the important part of the immune system in fish. This study was investigated the effect of singular or combined administration of Pediococcusacidilactici and Raffinosonmucosal immune parameters and intestinal histomorphology of gold fish (Carassiusa uratus). Material and Methods: 180 gold fish, with average weight of 26.3±0.18 after two weeks of adaptation to laboratory condition In 4 treatments and each treatment with 3 replicates including: commercial diet (control group), commercial diet supplemented with0.9 g kg-1P. acidilactici, 10 g kg-1Raffinosor simultaneously supplemented with 0.9 g kg-1P. acidilacticiand 10 g kg-1Raffinos accidentally divided. The trial lasted 60 days. At the end of the experiment, the lysozyme enzyme was measured by turbidation method using spectrophotometer and total immunoglobulin by measuring serum protein before and after adding polyethylene glycol to the sample and the amount of alkaline phosphatase enzyme using the kits produced by Pars Co. and using spectrophotometer, it was calculated. Histomorphologic tests of the intestine were done by classical histology and staining of hematoxylin-eosin. The results showed no significant difference in height and diameter of vili (P <0.05). The highest activity of mucosal lysozyme activity, immunoglobulin level, soluble protein and alkaline phosphatase were observed in synbiotic treatment, however, this difference was not significant in different treatments (P> 0.05). In conclusion, the commercial diet supplemented with 0.9×107 CFU/gP. acidilactici and 10 g/kg Raffinosimprovesmucusal immune para meter sand intestinal histo morphology of golden fish.