In vitro anti-bacterial effect of nano-polyamidoamine-G5 dendrimer
Message:
Abstract:
Introduction
Progress in nanotechnology in the past decayed has created various opportunities for evaluation of biological effects such as anti-bacterial effects of nanoparticles. This study was aimed to examine synthesis and the antibacterial effect of Nano-Polyamidoamine-G5 (NPAMAM-G5) dendrimer on Klebsiella Pneumoniae, Pseudomonas Aeruginosa, Shigella Dysenteriae and Bacillus Subtilis.
Materials and Methods
NPAMAM-G5 dendrimers was synthesized by Tomalia’s divergent growth approach. The antibacterial effects of NPAMAM-G5 dendrimer were studied by disc diffusion and micro-dilution method. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against gram-positive and gram-negative bacteria were determined according to Clinical and Laboratory Standards Institute (CLSI) guideline. Transmission electron microscopy (TEM) was used to analyze morphology and size of NPAMAM-G5.
Results
Zone of inhibition in concentration 25μg/ml of NPAMAM-G5 dendrimers for Klebsiella Pneumoniae, Pseudomonas Aeruginosa, Shigella Dysenteriae and Bacillus Subtilis were 27, 13, 30 and 18 mm, respectively. There was a significant difference regarding the zone of inhibition between gram-negative and gram-positive bacteria (p<0.05). Remarkably, the MIC for Klebsiella Pneumoniae, Pseudomonas Aeruginosa and Shigella Dysenteriae was 2.5μg/ml and for Bacillus Subtilis was 25μg/ml. The MBC for Shigella Dysenteriae and Pseudomonas aeruginosa were 50 and 200 μg/ml, respectively and for Klebsiella Oxytoca and Bacillus Subtilis was100 μg/ml. It was found that NPAMAM-G5 particles had a spherical shape with a mean diameter size of 10 nm.
Conclusion
According to the results, the NPAMAM-G5 dendrimer with end amine groups displayed a positive effect on the removal of standard strains of gram-positive and gram-negative bacteria.
Language:
Persian
Published:
Pages:
171 to 180
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