Identification the Growth Hormone Gene Complete Sequence and Phylogenic Analysis of Sterliad Sturgeon (Acipenser Ruthenus)

Growth Hormone is a single-chain polypeptide that promotes somatic growth, development and metabolism in vertebrates. In this study the starlet sturgeon growth hormone gene was isolated and sequenced via cDNA technique. In first, four pituitary gland of starlet sturgeon were isolated and RNA purification performed with BIOZOL solution. Then, by using cDNA and PCR techniques the growth hormone gene sequence was obtained. Results showing that the nucleotide sequence of starlet sturgeon is 768 bp. The results showed that the nucleotide sequence of Persian sturgeon growth hormone is 768 bp including 26 bp 5’’ untranslated region and 98 bp 3’’ untranslated region with an open reading frame of 645 bp. Comparing the starlet growth hormone gene with other vertebrates shows the highest similarity with mammals (64-67%) followed by anguilliformes, amphibians and other teleosts. The high level of similarity between the starlet growth hormone gene sequence and mammalian species suggests that GH is derived from a common ancestral gene.