Preparation and Evaluation the Effects of Microemulsion Containing Honey and Sesame Oil on Candidate Sepsis in Mouse

Sesame oil has anti-candidiasis therapeutic effects. Due to hydrophobic property of oils, their solubility in aqueous media is low which causes their low absorption and impact on the reduction of microbe compactness in the body. The aim of the present study was preparation of a mixture containing sesame oil and honey based on microemulsion form. An experimental study was conducted. Tween 80 as surfactant and honey as co-surfactant with various proportions of surfactant/co-surfactant as 4:1 ,2:1, and 5:1 were selected to plot pseudo ternary phase diagram. Microemulsion preparation was performed based on the titration method. An in vivo study was performed on 12 BALB/C mice which were divided into two groups. Each group had six mice as the control group and drug consumer group (microemulsion including honey and sesame oil). Physicochemical properties of the microemulsion samples showed that the mean particle size is in the range of 24.09-16.62 nm with a homogeny particle size distribution between 0.33-0.28. Zeta potential was in the range of 16.5-10.3- and refractive indices and conductivity coefficient were about 1.39 and 279-249 μS, respectively. These results confirm the formation of oil in water microemulsion due to calculated refractive indices near water value, higher value of conductivity coefficient, and small particle size. Stability test during time showed that samples with 2:1 surfactant to co-surfactant ratio were not stable after two months. Samples with 4:1 surfactant/co-surfactant ratio were selected for in vivo evaluation due to higher stability and oil content. It seems that using emulsion is effective in contamination with fungi in blood culture and increased peritoneal macrophages in mice.