Effects of Commercial Dietary Prebiotic (Agrimos®) and Probiotic (Batocell®) Supplements on Intestinal Lactic Acid Bacteria, Salinity and Thermal Stress Resistance in Zebrafish (Danio rerio)

Present study investigates the effects of two commercial dietary prebiotic and probiotic supplements on thermal and salinity stress resistance and intestinal Lactic Acid Bacteria (LAB) population in zebrafish. A total of 630 healthy 15 days old zebrafish were distributed in 21 aquariums and assigned to 7 groups fed control (T0) or diet supplemented by 2, 4 and 8 g kg-1Agrimos® (T1, T2& T3) and 0.1, 0.2 and 0.4 g kg-1Bactocell® (T4, T5& T6) for 90 days. At the end of the experiment, the results of prebiotic treatment showed significant (P<0.05) increase in salinity stress resistance treated fish to the control group during the experimental period. In thermal stress resistance, all groups showed positive results compared to control one. Lactic acid bacteria (LAB) count in Prebiotic and probiotic treatments was significantly higher than control group (T0), but in total bacterial count, probiotic treated fish showed significant difference with other groups. The result demonstrated that these commercial dietary supplements have beneficial effect on the assayed parameters and can improve stress resistance and modulated intestinal microbiota of zebrafish.