Investigation of Polymorphism in 5- Flanking Region of Pit-1 gene and its association with growth traits in Iranian native Goose

The objective of this study was detection of polymorphism in 5- Flanking region of Pit-1 gene and its association with growth traits in Iranian native Goose by PCR-SSCP method. For this purpose, blood samples were taken from 160 Goose from East Azerbaijan breeding station that located in Malekan. DNA was extracted from whole blood using the Bailes method and polymerase chain reactions were performed for amplification of 241 bp fragment containing in 5- Flanking region of Pit-1 gene.Single Strand Conformation Polymorphism (SSCP) was used for genotyping. For this purpose, vertical electrophoresis of PCR products was performed on 8% acrylamide gel, at 120 V, for 18 h at 4 C˚. Silver-staining of gels is used to detect, resulted four genotypic patterns of 1, 2, 3 and 4 with frequencies of 31.2%, 36.4%, 7% and 25.4%, respectively. Analysis of variance was performed using GLM proc of SAS software. The statistical analysis indicated that the effect of different patterns were significant on the 30-Day Bodyweight (p<0.01), but on the 60-90-150 -Day Bodyweight had no significant effect.