Phylogenetic and genetic diversity of Spirlin fish(Alburnoides eichwaldi) based on cytochrome b sequencing in five rivers of the Northern Areas in Iran

Spirlin Alburnoides eichwaldi(De Filippi) a small fish of the Cyprinidae, Bentoplagic Anadromous, which lives in freshwater and brackish and can bear pH range 7-8. Mainly in distributed in shallow area and mountain streams with hard beds and prefer clean and well oxygenated. The importance Spirlin in the fishery, usually as bait for recreational fishing and the aquarium is maintained. Due to the small size and economic value of sport fishing and fisheries, but considering the beautiful colors on the body is an aesthetic value. In These study, Phylogenetic and genetic diversity of Spirlin fish (Alburnoides eichwaldi) using cytochrome b sequencing in five rivers Zringl, Madarsoo, Safarood, Klisiyan, Zalki of the Northern Areas in Iran. Total of 26 specimens of spirlin collected and transported to the laboratory and DNA was extracted from the tissue. The results of the nucleotide sequences specimens showed Haplotype diversity is good, and 17 haplotypes was identify which Indicated the high genetic diversity These fish in rivers. Also based on Phylogeny analysis are three clades of spirlin in rivers. The present mitochondrial DNA data suggest that the southwestern populations in the Sefidrud drainage basin includes rivers and Zalki and Safarood (clade I) may be considered as A. eichwaldii, whereas the kelisiyan river populations (clade II) might be a distinct taxon of Alburnoides sp1. The clade of eastern populations (Gorganrud drainage basin, clade III) namely, Madarsoo and Gorganrud, are clearly separated from that of the kelisiyan (clade II) and Sefidrud (clade I) drainage basins, making a distinct species (Alburnoides sp2).