The study of changes in 17β-estradiol and 17α-hydroxyprogeterone levels of plasma by administration of dopaminergic and adrenergic agonist & antagonists with GnRHa and Ovaprim in Caspian Kutum Rutilus frisii kutum

Caspian Kutum (Rutilus frisii kutum Kamenskii, 1901), is one of the most valuable commercial and endemic fish in the Caspian Sea. The aim of the present study was to evaluate the effects of the third generation dopaminergic pharmaceutical compounds antagonists and adrenergic agonist and antagonist pharmaceutics with Buserelin (GnRHa) on plasma levels of 17β-estradiol (E2) and 17α-hydroxyprogeterone (17α-OHP) in this species. 117 gravid female were selected for the study, pharmaceutics including, Buserelin Acetate (BUS) 5 μg kg-1 BW, Ovaprim, 0. 5 ml kg-1 BW, Salbutamol Sulphate (SLB) 4 mg kg-1 BW, Olanzapine (OLZ) 5 mg kg-1 BW, Clozapine (CZ) 12 mg kg-1 BW و Metoprolol Tartrate (MTP) 5 mg kg-1 BW. Pharmaceutics were divided into 10 groups: Group 1: OLZ+SLB, Group 2: OLZ+MTP, Group 3: OLZ+BUS, Group 4: CZ+SLB, Group 5: CZ+MTP, Group 6: CZ+BUS, Group 7: OLZ+BUS+SLB, Group 8: OLZ+BUS+MTP, Group 9: CZ+BUS+SLB, Group 10: CZ+BUS+MTP. Saline 0. 7% and Inact were considered as the positive and negative Control respectively, also Ovaprim was the evidence treatment (n=9).
The results showed that the level of E2 was increased before administration of pharmaceutics in 0 h, but was decreased followed by pharmaceutics administration (12 h), so that Ovaprim, OLZ+BUS+SLB and CZ+BUS treatments were significantly differed compared with other treatments (P<0. 01). Also the treatment OLZ+SLB, OLZ+MTP, CZ+SLB, OLZ+BUS+MTP and CZ+BUS+SLB were significantly differed in P0. 05). The level of 17α-OHPwas low before pharmaceutics administration in 0 h, but was increased after administration of pharmaceutics (12 h). Ovaprim, OLZ+BUS+SLB, CZ+BUS and OLZ+SLB treatments were significantly differed in compared with other treatments in 12 h (P<0. 01). it could be concluded that, dopaminergic system was inhibited reproductive functions while adrenergic system stimulated it, thus there was an inverse relationship between the dopaminergic and adrenergic systems. So that adrenergic system agonist can be used beside the third generation Dopamine antagonists and GnRH analogue in Caspian Kutum.