Effects of various concentrations of animal derived ingredients-free extenders on sperm parameters of goat semen during liquid storage at 4°C

One ml of semen obtained from 4 Mahabadi goats (3- to 4-year-old) during reproduction session was mixed with 20 ml of a tris buffer based extender contained 0.5, 1, 1.5, 2 and 2.5 % soy lecithin as a non animal-derived additive or 1 % egg yolk as an animal-derived additive and stored in 4°C. The total and progressive motility, viability, membrane integration, abnormal morphology of the sperm at 2, 6, 24 and 48 h after storage were assessed. Results showed that sperm motility and memberan integration decreased with increasing of storage time (P<0.05) and there were no different between different extender for these parameters (P>0.05). At 24 h after storage in 4°C sperm viability in the 1.5% soy lecithin-containing extender was higher than those exposed to the 0.5% soy lecithin-containing extender. At 6, 24 and 48 h after storage in 4°C the percent of sperms with abnormal morphology was lower in 1.5% soy lecithin-containing extender than other soy lecithin-contained extenders or egg yolk-contained extenders(P<0.05). Therefore, 1.5% soy lecithin-containing extender is a proper extender for storage goat semen at 4°C.