Feasibility study of use of Rana ridibunda toe bone as a bioindicator for monitoring of heavy metals (Pb, Cd, Ni and V) pollution in Qarasoo River water

Due to discharge of urban, agricultural and industrial wastewater to Qarasoo River, there is a possibility of accumulation of pollutants in water, sediment and aquatic animal tissues. Therefore, this study was conducted to survey of use of Rana ridibunda toe bone as a bioindicator for monitoring of heavy metals (Pb, Cd, Ni and V) pollution in Qarasoo River waterin 2014. Water and frog samples were collected from 4 selected stations. After laboratory preparations of samples, the concentrations of elements were determined by ICP-OES in them. All statistical analyses were performed using the SPSS statistical package. The results showed that significant correlation between mean concentration of heavy metals in water samples and adults and tadpole's toe bone samples. Also no significant difference was found between tadpoles and adults toe bone samples (P>0.05). Studying different tissues in bioindicator specimens can demonstrate the changing trend of pollutant concentration in aquatic systems. In summary, the elemental analysis of toe clipped bone samples have several advantages: found significant correlation between mean concentration of metals in water and toe bone, there is no need to kill the animals, the analytical procedure has a small chemical reagent demand, and only a small amount of tissue is sufficient to make several kinds of laboratory.