Evaluation effect wheat germ oil on sperm parameters, hormone testostrone and Lipid peroxidation following treatment with carbamazepine in epileptic male mice with PTZ

Epilepsy is a neurological disorderwhich appears with impairment of brain electrical activity. Carbamazepine is an antiepileptic drug that causes oxidative stress and disorders in reproductive system in long-term. The purpose of this article was to investigate the antioxidant effect of wheat germ oil (WGO) on testosterone, sperm parameters and Lipid peroxidation levels of carbamazepine-treated epileptic rats. In this study 48 adult male rats were randomly divided into 6 groups (n=8), including:1. Control; 2. Pentylenetetrazole(PTZ) with a dose of 40(IP); 3.PTZ 40 + CBZ at a dose of 30(gavage); 4. PTZ 40 + CBZ with a dose of 180(gavage);5.PTZ (40) + CBZ at a dose of 30 + WGO at a single dose of 500(gavage);6. PTZ + CBZ with dose 180 + WGO with a single dose of 500 (dose unit= g/kg body weight). At the end of the treatment period (42 days), serum testosterone levels, MDA and sperm parameters were measured. Data were analyzed by one-way ANOVA and Tukey test using SPSS20 software. In this study, in comparison to control group, testosterone level and sperm parameters of groups receiving CBZ were reduced (p<0.05). Also, lipid peroxidation levels increased in these groups (p<0.05). The use of wheat germ oil caused significant increase in testosterone and sperm parameters and decreased lipid peroxidation level compared to the carbamazepine - receiving groups. The results showed that wheat germ oil improves reproductive system performance and reduce the side-effects of carbamazepine desirably.