Effects of different levels of Pediococous acidlactici in the diet on survival rate of broodstocks, cyst and naupli production, immunological enzymes and coloy count of digestive tract of Artemia franciscana

The effects of probiotic effects of (Pediococous acidlactici) on survival rate of broodstocks, cyst and naupli production, immunological enzymes and coloy count of digestive tract of Artemia franciscana were examined. Three diets containing 102 (T1), 104 (T2), 106 (T3) CFU of probiotics g feed-1 and a control diet (C) without probiotic were used daily through a completely randomized design (each with three replicates). The results showed a significantly (P<0.05) increment in the number of cyst and produced naupli with Artemia franciscana and also the survival rate of broodstocks compared to those of control. The highest number of cyst and naupli was obtained in the T2 (215 l-1) and T3 (1909 l-1), respectively. The immunological enzymes of Artemia franciscana fed the experimental diets did not show significant differences with those of control group but Artemia fed the T2 showed the highest activities of lysozyme (4.15 U µg-1), phenoloxidasse (2.74 U min-1) and superoxide dismutase (2.90 U min-1) while the highest activity of catalase (2.79 U min-1) was for T3. The colony count (CFU ml-1) of Pediococous Acidlactici formed in the digestive tract of artemia showed a significant difference with that of control group and the highest count was observed for T1 (760). Based on the results, the proposed range of probiotic in the diet of Artemia franciscana was 102-104 CFU g-1.