Investigation of two phenotypes of the Gorgani Cave Salamander (Paradactylodon gorganensis) Using D-loop Region Sequencing

The Gorgani Cave Salamander (Paradactylodon gorganensis) is one of at risk species that only habitat of this species is a cave in Shirabad of Golestan province. In order to genetic determination of two phenotypes of Gorgan Mountain Salamander, after sampling and DNA extraction from ventral fins of 10 Salamander, the D-loop region was used. After loci amplifying in PCR, the all samples were sequenced. In this study, the results revealed that all sequence pattern of D-loop region were similar that it can be concluded that The D-loop region in the two strains was a one haplotype and there wasn’t any variation and it would appear that the high genetic similarities and no differences are in the two phenotypes of this population.