Investigation of Phenolic Compounds Exuded from Single Node Microcuttings of Walnut into Liquid Culture Medium DKW

In order to study the amounts of some phenolic compounds exuded from single node walnut explants in liquid medium (DKW), this experiment was performed in the form of a split units in time experiment based on split block design with three factors including cultivar in two walnut cultivars namely Chandler and Jamal, time of sampling in four levels including May 5 th, May 21st, July 5th and September 5th and the time of liquid culture assessment in three levels (24, 72 and 144 hours after the culture) using HPLC with three replicates. In independent experiment, phenolic compounds exuded in liquid medium 24, 72 and 144h after the culture and juglone were added to solid medium. The results showed that in terms of the 14 phenolic compounds (Ellagic, Vanillic, Coumaric, Chlorogenic, Caffeic, Gentistic Ferulic Syringic Cinamic acid, Catechin, Rutin and Myricetin, Juglone and 1,4 Naphthoquinone) in shoots and medium the cultivars were not significantly different but the time of sampling and liquid culture assessment showed significant differences between the cultivars. The results showed that Juglone, Myricetin, Ellagic and Vanillic acid were present in the current year shoots in higher amounts than other phenolic compounds and Juglone, Ellagic acid and Myricetin had the highest leakage in the culture medium. Adding exuded phenolic compounds after 144h in the solid medium had the lowest growth and fresh weight change of microcutting and significant difference with other treatments.The over all results of this study showed that Juglone, Ellagic acid and Myricetin are possibly the main obstacles to the fully succeed microcuttings establishment of two Jamal and Chandler cultivars.