Expression of VEGF and TGF-β Genes in Skin Wound Healing Process Induced Using Phenytoin in Male Rats
Message:
Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
Background
Phenytoin is one of the most important chemical drugs used for the treatment of skin wound.
Objectives
In the present study, the molecular effect of phenytoin on the expression of VEGF and TGF-β genes was investigated.
Methods
This study was conducted on 30 male rats with approximately equal body mass. Skin wounds were generated with an area of 2 cm and a depth of 0.5 mm on the back of the necks. The rats were divided into two case and control groups (15 rats in each group). Topical phenytoin was administered to the two groups 2 times a day (1%) and Vaseline (as control group). Each of control and treated groups were divided into three subgroups and the rats were euthanized with chloroform on days 7, 14, and 21 of post-wounding. The wounds were harvested from control and treated rats. After homogenization of the tissues, RNA was extracted, purified, converted to cDNA, and the relative expression of VEGF and TGF-β genes in phenytoin and control groups was evaluated by real-time PCR. The gene expression was evaluated on days 7, 14, and 21.
Results
Increased expression of VEGF and TGF-β in the first week and decreased expression of both genes in the third week were observed in the phenytoin-treated group compared with the control group (P < 0.001).
Conclusions
Regarding the results of the expression of both TGF-β and VEGF genes, there is a significant relationship between the expression of these two genes and the rate of wound healing in rats.
Language:
English
Published:
Jundishapur Journal of Health Sciences, Volume:11 Issue: 1, Jan 2019
Page:
6
magiran.com/p1932922  
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یک‌ساله به مبلغ 990,000ريال می‌توانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
  • حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران می‌شود.
  • پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانه‌های چاپی و دیجیتال را به کاربر نمی‌دهد.
دسترسی سراسری کاربران دانشگاه پیام نور!
اعضای هیئت علمی و دانشجویان دانشگاه پیام نور در سراسر کشور، در صورت ثبت نام با ایمیل دانشگاهی، تا پایان فروردین ماه 1403 به مقالات سایت دسترسی خواهند داشت!
In order to view content subscription is required

Personal subscription
Subscribe magiran.com for 50 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!