The study of the effect of hydroalcholic extracts of broccoli induced oxidative stress by lead in kidney of mice

Considering the abundance of lead in ecosystems, the aim of this study was to investigate the effect of hydro alcoholic extract of broccoli on lead acetate oxidative stress in kidney of mice. In this study, 40 albino mice with 30±5 g weight were divided in 4 groups. Control group received 500 ppm lead in running water while mice in T2, T3 and T4 groups, in addition to 500 ppm lead acetate in running water, received intraperitonealy 100, 200 and 300 mg/kg of hydroalcoholic of broccoli extract, respectively. Blood samples were taken from the animals. After being euthanized the right kidney was fixed in 10% formalin buffer for pathological studies and Malondialdehyde (MDA) was measured in the left kidney. A significant total weight and kidney weight increase was observed in control group. The concentration of MDA in control, T1, T2 and T3 groups were measured as 34.67±2.56, 27.98±3.056, 16.86±4.654 and 12.06±6.765 µmol/ml, respectively. A significant difference was observed between control and T2 and T3 groups. The highest concentration of Total Antioxidant Capacity (TAC) was observed in T3 group with 1034±223.121 and the least concentration was in control group 709.70± 35.34 µmol/ml respectively, which showed a significant difference between the control group and T1, T2 and T3 groups. In the histopathologic studies, the highest damage rated 7 was seen in the control group and the lowest was observed in T3 group. According to the results, it can be concluded that broccoli hydroalcoholic extract at 300 mg/kg dose controls the effects of lead acetate oxidative stress in kidney of mice.