In-Silico ‌‌‌‌‌‌‌‌‌‌‌‌EST analysis of rice genes induced by Benzothiadiazole treatment

Induced resistance (IR), activated either by a prior pathogen infection (biological induced resistance) or by treatment with a chemical. Induced resistance is thought to play an important role in the preservation of plants in nature. Although several types of IR have been identified, the one that has been studied most extensively is systemic acquired resistance (SAR). Systemic acquired resistance is an inducible defense mechanism that plays an important role in disease resistance in plants. It has been found that agrochemical components cause SAR in plants. One of the effective agrochemicals is benzothiadiazole (BTH). The exogenous application of BTH to leaves augments the sensitivity of cells to respond to elicitation. BTH has been developed as a novel disease control compound that is translocated throughout the plant where it acts by inducing an inherent disease resistance response. In order to investigate the mechanisms of BTH biochemical activity and its induced genes, BTH-related EST sequences were mined from NCBI site. Clustering and assembling about 370 rice EST sequences were formed after treatment with BTH resulted in 33 contig and 234 singltone, and then functional groups were determined and compared with the two libraries of common genes. The results of this study indicated that after application of BTH on rice, kinase, LHCB and OEEP proteins as well as the carbonic anhydrase and Ribulose enzymes are expressed. This study is the first report that effect of BTH on plant photosynthesisc. In this study novel candidate genes were identified which may have a possible involvement in plant defense mechanism. This kind of bioinformatics studies may be useful in molecular breeding programs in rice for development of diseases tolerance varieties.