Assessment of the anti-inflammatory activity of the combination of deuterium depleted water and Rosa damascena Mill. against septic injury in rats

Sepsis is the most common reason of mortality among patients who are in the intensive care unit. Regarding to the side effects of the anti-inflammatory drug consumption, the replace of natural products are suggested in sepsis treatment. the effects of the combination of the deuterium depleted water (DDW) and Rosa (R.) damascena Mill. on the stress oxidative parameters and the gene expression of cyclooxygenase-2 (COX-2) in the lung and plasma tissues were investigated. 50 Rats were randomly divided into 5 groups: negative control (LAP), control group (CLP), two treatment groups with the combination of DDW and R. damascena Mill. essential oil (DDW15+EO and DDW30+EO) and positive control group with indomethacin (IND). Then, the levels of oxidative stress parameters and the expression of COX-2 were estimated in plasma and lung tissue. The sepsis resulted in the decrease of ferric reducing antioxidant power (FRAP) and glutathione (GSH) levels along with the increase of lipid peroxidation (LP) and COX-2 levels. However, the rats treated with the combination of deuterium depleted water and R. damascena Mill. essential oils as same as indomethacin were influenced on the regulation of those parameters through the evaluation of FRAP and GSH levels and the reduction of the LP level and COX-2 gene expression. The pathological studies confirmed the biochemical consequences as well. The results indicated that the oxidative damages were caused by sepsis, but the administration of the natural products such as deuterium depleted water and R. damascena Mill. essential oils could improve the injures due to the effectiveness of oxidative stress and antioxidants parameters.