The effect of short-term training and curcumin on the paraoxonase-1 activity after alcohol withdrawal in male Wistar rats

  The paraoxonase-1 (PON-1) which is synthesized in the liver is an enzyme linked to HDL. It acts as an antioxidant as well as anti-inflammatory agent, and in the presence of calcium, it hydrolyzes free radicals and prevents LDL from oxidation. Alcohol consumption and sedentary lifestyle causes fat disorder and a decrease in the PON-1 activity. The purpose of this study was to determine the effect of training and curcumin on the PON-1 activity and lipid profile after alcohol withdrawal in male Wistar rats. For this study, 32 male Wistar rats were divided into four groups based on alcohol consumption: 1) alcohol, 2) alcohol + training, 3) alcohol + curcumin, and 4) alcohol + training + curcumin. After four days of alcohol consumption program and six days of alcohol withdrawal, curcumin and training intervention (swimming) was performed for fourteen days. Blood samples were collected for laboratory analysis. In order to investigate inter-group differences, a two-way analysis of variance and the LSD post hoc test was used. Independently, the training increased the activity of the PON-1 (p=0.001). Further, the interaction of exercise and curcumin had a significant increase in the activity of PON-1 (p=0.02). Moreover, the training independently increased the concentration of HDL (p=0.01). Additionally, the interaction of exercise and curcumin showed a significant effect on the increasing of HDL (p=0.01). The findings of this study confirm that combining short-term training of swimming and curcumin increases the activity of PON-1 and the concentration of HDL in the male Wistar rats. Therefore, in order for reducing the complications of alcohol withdrawal period, we can use training + curcumin in improving lipid profile and increasing PON-1 activity.