Evaluation the effect of different solvents on extraction yield and antioxidant activity of Eryngiumcaucasicum Trautv leaf extract
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Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
In the present study, the effect of different solvents on extraction yield and antioxidant activity of Eryngium caucasicum Trautv. extract were investigated. Soxhlet method was used for extraction of antioxidant compounds. Extractions were performed using methanol, ethanol, and hexane at three different levels of extraction time including 2, 4, and 6 hours. The results showed that the highest extraction yield could be achieved using ethanol during 6 h of extraction time (52.00±0.26%), while the lowest value was obtained using hexane during 2 h of extraction time (6.72±0.21%). The antioxidant activity was measured using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydrogen peroxide (H2O2) assays. The highest value of antioxidant activity measured using DPPH assay was determined for the methanolic and ethanolic extracts using 6 h of extraction time (29.21±0.19%, 28.27±0.25%, respectively) with insignificant (p>0.05) difference. The lowest value of antioxidant activity was belonged for the extract obtained using hexane during 2 h of extraction time (5.90±0.16%). According to the results of H2O2 assay, the highest and the lowest antioxidant activity was determined for the extracts obtained using ethanol during 6 h of extraction time (14.15±0.22%) and hexane during 2 h of extraction time (0.8±0.2%), respectively. Finally, according to the results obtained it could be concluded that extract obtained using ethanol during 6 h of extraction time showed the highest values of extraction yield and antioxidant activity compared with the other extracts.
Language:
Persian
Published:
Food Science and Technology, Volume:15 Issue: 10, 2018
Pages:
379 to 386
magiran.com/p1936993  
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