Effect of dicalcium phosphate dihydrate bioceramic nanoparticles on the electrospun poly) ε-caprolacton (nanofibers for bone tissue engineering

Poly (ε-caprolactone) (PCL) / Dicalcium phosphate dihydrate (DCPD) composite nanofibers were prepared in an appropriate solvent of chloroform / methanol (75:25) by electrospinning method. The results of the scanning electron microscope showed uniformly diameter of fiber and bead-free structure by changing the electrospinning parameter. Elemental mapping results confirmed the presence and proper distribution of DCPD nanoparticles into PCL matrix. The results of measuring the contact angle represents the reduction of the contact angle of scaffold with average diameter higher compared with an average diameter less. Bioassay (cell survival) was performed by MTT assay and the lack of toxicity of the scaffolds for human gingival cells (HGF) was revealed. Also, better wettability and hydrophilicity have provided more favorable conditions for adhesion, growth and proliferation of cells, and more biocompatible composites have been obtained. These results indicate that PCL-DCPD composite scaffold can provide a suitable substrate for the growth of bone cells.