A survey of high-level resistance to gentamicin and aminoglycoside-modifying enzyme-encoding genes in Enterococcus faecalis and Enterococcus faecium isolated from infected burn wounds

Aim and Background: The aim of this study was to survey of High-level resistance to gentamicin and aminoglycoside-modifying enzymes (AMEs) encoding genes in Enterococcus faecalis and Enterococcus faecium isolated from burn wound infection. Antibiotic susceptibility test was performed after identification of E. faecalis and E. faecium strains. In order to detect HLGR strains, gentamicin disks were used. PCR test was performed to identify the AMEs-encoding genes. Of 114 isolates of Enterococcus, 56.1% of the strains were HLGR. All HLGR strains have the aac (6') -Ie -aph (2'') -Ia gene. 1.9% of the HLGR E. faecium strains carried the aph (2 ") -Id gene. Our data showed that a high prevalence of resistance to gentamicin in burned wound infection in our hospital. The main responsibility for the resistance to gentamicin in the present study was the aac (6 ') -Ie -aph (2'') -Ia gene.