Study of Abundance and Genotyping of Human Papillomavirus in Samples of Patients Suspected to Cervical Cancer Referred to Masoud Labratory in Tehran

Cervical cancer is the second most common cancer in women worldwide. The presence of HPV has been implicated in more than 99% of cervical cancer worldwide. Human papillomavirus is a heterogeneous virus, categorized to two groups of high-risk and low-risk genotyped according to the level of infection that leads to cervical cancer. HPV screening is recommended for the further evaluation of abnormal pap test or during follow-up after treating precancerous lesions. Genotyping of different high-risk HPV (hrHPV) types obtained from smear tests has not yet gained widespread acceptance in clinical practice. Genotyping of hrHPV could be helpful for the risk stratification of HPV-positive. In this study 143 women residual samples were available for HPV assays in Masoud laboratory in Tehran. After extracting DNA by kit, for genotyping detection of human papillomavirus PCR amplification, Reverse dot blot hybridization methods was used. Among these samples, 34.2% were HPV positive. In this research, the most common genotype was HPV-18 from high risk HPV genotype and HPV-6 was the most common from low risk genotype of HPV. The most Age category that has been detected positive HPV from category 26-35 years. The variety of HPV genotype detection in cervical cancer screening increases the importance of epidemiological study controversially in world. Therefore for characterizing genotype of HPV, Reverse dot blot hybridization can be of great help in this regard.