The Prevalence of Papillomavirus-16 and -18 isolated from women with cervical cancer using Multiplex PCR

Women infected with human papillomavirus, especially types 16 and 18, are at risk of cervical cancer. The purpose of this study was to determine the frequency of papillomavirus-16 and -18 in women with cervical cancer using multiplex-PCR. In this experimental study, after collecting blood samples, viral DNA was extracted using a Cinaclone kit, and PCR, with specific primers, was performed to detect HPV-16 and HPV-18. PCR products were analyzed by 1% agarose gel electrophoresis. Of 60 samples, 19 were infected with HPV. The results showed that the frequency of genotype HPV-16 and HPV-18 was 8 (42.1%) and 11 (57.9%), respectively. The study showed that using PCR with specific primers for the detection of HPV-16 and HPV-18 is a convenient and accurate method. The results of this study indicate the relationship between HPV and cervical cancer.