Antinociceptive effect of Salvia Sharifii Rech. F. & Esfand seed's oil in formalin test in mice

Salvia sharifii Rech. F. & Esfand (Lamiaceae) is an Iranian endemic plant that widely used as a medicinal plant. Due to the antinociceptive and anti-inflammatory effects of other Salvia species, the present study was conducted to investigate the antinociceptive effect of S. sharifii seed's oil (SSSO) in experimental animals. Seed's oil of S. sharifii was extracted by Soxhlet method. In order to assess the antinociceptive activity, the animals were classified to five groups: control (vehicle), S. sharifii seed's oil (20 and 100 mg/kg) and standard groups (morphine 5 mg/kg and mefenamic acid 30 mg/kg). The analgesic activities were studied by formalin test in mice. The nociceptive responses were divided to two phases; phase I (0-15minutes) and phase II (15-60 minutes). Comparisons between the groups were carried out using the analysis of variance (one way ANOVA) and post hoc Tukey's test. P<0.05 was considered as significant difference. S. sharifii seed's oil showed significant antinociceptive effects in first phase (dose 20 mg/kg) and second phase (dose 20 and 100 mg/kg) of formalin test compared to control group. These effects were comparable to morphine and mefenamic acid in chronic phase of formalin test. The results indicated that S. sharifii seed's oil induced a significant antinociceptive in mice that is likely related to active constituents of this seed's oil.