Comparison of the Antimicrobial Properties of Mmethanolic Extracts and Essential oils of Trachyspermum capticum on E.coli and Enterococcus faecalis
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Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
Background and objective
Trachyspermum capticum plant is from Apiaceafamily of essential oil and seed extract of this plant contain compounds that show its biological properties. The aim of this study was to investigate and compare the antimicrobial activity of essential oil and methanolic extract of herbs.
Materials and methods
The chemical compounds of this medicinal plant were analyzedby GC-MS and 7 components were identified, representing 85% of its total essential mass. The antibacterial properties of these agents were evaluated by determining the minimum inhibitory concentration (MIC) and minimum inhibitory concentration (MBC) against E. coli and Enterococcus faecalis bacteria with microdillution method, and the functional groups of the compounds were investigated using FTIR analysis.
Result
Results showed that the EO Trachyspermum capticum was formed by the main components of (Thymol 30%), (Limonene 21%) and (gamma-terpinene 19%). Factor groups derived from the resulting curves indicate that the flavonoid compounds in the extract have been shown to have antioxidant and antimicrobial properties. The results for the methanolic extract (MIC) were 15.62 mg/ml against enterococcus faecalis bacterium and 31.25 mg/ml against E.coli and MBC 125 mg/ml against enterococcus faecalis and 5.62 mg/ml is obtained against Escherichia coli bacteria. MIC and MBC of this species were found against Enterococcus faecalis 5 mg / ml bacteria and against E. coli (0.625mg/ml).
Conclusion
Essential oils and its methanolic extract play an important role in antimicrobial activity and its essential oil is much stronger than its extract. The presence of flavonoids in this plant is due to this biological property.
Language:
Persian
Published:
Iranian Journal of Infectious Diseases, Volume:23 Issue: 82, 2018
Pages:
15 to 24
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