Effect of hypercholesterolemia on the distribution of peripheral T lymphocyte activation markers

  The correlation between high levels of blood lipid with the induction of some diseases indicates significant effects of hyperlipidemia and especially on the immune system, inflammatory response, and secretion of cytokines. This is due to changes in the composition of cholesterol in the cell membrane and macrophage cytoplasm, which disrupts the signaling pathway necessary for the innate immune response. The purpose of this study was to investigate the effect of on phenotype properties of T cells and the expression of its associated activation markers. In the present experimental study 3ml of peripheral blood samples were collected from 30 patients and 30 healthy subjects. The distribution of activation markers was evaluated by Immunophenotyping with anti-CD4, CD8, CD25, and CD69 antibodies. was used and output data were analyzed using Flow Jo 10 and SPSS 16 software. Evaluation of the activation markers located T cells of patients with showed a significant decline by 0.8% and 2% in the expression of CD25 marker and 1.92% and 2.12% in the expression of CD69 marker on CD8+ CD4+ T cells, respectively (p<0.05). The changes in the phenotype properties of T cells and the decreased expression of activation markers in high-level cholesterol conditions might weaken the immune system in hyperlipidemia patients.