Investigation on genetic diversity of Epeorus along Gamasiab River in different seasons

This study was conducted to determine the profile of Epeorus genetic diversity along the Gamasiab River. The sampling was done from four stations ranged source of the river downward, about 500 meters from each other, in four seasons with three replications from the middle and riverside of the river by the Surber sampler. After isolation and fixation of the Epeorus, the DNA extraction steps were performed and the quality of DNA was investigated on the electrophoresis gel. After gene amplification using specific cytochrome oxidase subunit I (COI) primers, PCR products were sequenced. Sequencing was performed with MEGA 6 software. Molecular investigations indicated that the reduction of genetic diversity at station 2 exposed to wastewater and trout farm effluents, especially in the seasons which are associated with decreasing water charge.