Genetic Diversity of Bread Wheat Cultivars Using SSR Marker and Relationship Analysis for Two Traits Prolin and Fructan under Spring Chilling Stress

In this study, the genetic diversity of 22 bread wheat cultivars was investigated through microsatellite markers and physiological traits under controlled condition. For this purpose an experiment was implemented via those varieties with four treated levels of cold stress (8 (control), +2, 0, -2 Celsius) in factorial arrangement in a randomized completely block design (RCBD). Results of variance analysis showed the effects of interaction between cultivars spring cold levels is signification at the 1% probability of error level. According the results of trait Proline content in -2ºC, The highest level of this trait belongs to Moqane2 and the Golestan was the lowest one. Fructan content, Also in this level of cold stress, showed Ohadi and Dez cultivars, respectively. To investigate the genetic diversity at the molecular level were used from 21 microsatellite primer pairs. The 11 pairs of them could show good polymorphism and totally identified 19 alleles. The mean number of produced alleles was 2.27 alleles per marker locus and the range of alleles at loci was 1 to 4. The polymorphic information contents (PIC) values of the loci had ranged from 0.09 t0 0.98 with average 0.62. Results of stepwise regression, shows the significant relationship 2 markers, Xgwm174 Proline-related traits in intense stress condition and Xgwm642 with fructan-related In terms of control.