Evaluation of clove and tobacco extract and tricaine methane sulfonate (MS222) sedation on some of serum enzymes and biochemical parameters in common carp

In this study sedative effect of clove extract and tobacco extract and MS222 on some of serum biochemical parameters of common carp was studied. Two hundred ten fish were divided into 7 groups (two concentrations of clove extract 20 and 40 mg/l, two concentrations of Tobacco extract 40 and 80 mg/l and two concentrations of MS222, 20 and 40 mg/l for 2 days and one group considered as control). Each group had 10 fish with 3 replicates. At days 2, 4 and 6 after sedation blood sampling was done and some biochemical parameters including: total protein, glucose, cortisol and activities of serum enzymes AST, ALT, CPK and ALP were measured in plasma. According to results no significant differences in ALT and cortisol were seen between different experimental groups. The AST level in 40 mg/l tobacco extract group decreased significantly (P<0.05) in comparison with control group. ALP level in the clove extract groups and tobacco extract groups increased significantly (P<0.05) in comparison with control group. The level of total protein was decreased significantly by clove extract (40 mg/l) and tobacco extracts (P<0.05). The level of glucose in MS222 group (20 mg/l), clove extract groups and one of tobacco extract groups (80 mg/l) decreased significantly (p<0.05) in comparison with the control group. Although the use of clove or tobacco extracts at concentrations studied, made an increase in the activity of some serum enzymes, but there was no difference in the quality of sedation when compared with MS222.