Phylogenetic Evaluation and Molecular Variation of Leishmania major and Leishmania tropica Isolated from Different Parts of Iran

​ The aim of this study was species identification and analysis of species of Leishmania isolated from clinical samples. The samples were collected from patients that were infected from different parts of Iran. After microscopic examination, we used PCR method for the ITS1 (internal transcribed spacer 1) RFLP method (digestion with and for phylogenetic construction, DNA sequencing of Two samples from Khorasan province (Mashhad) were Leishmania (L. ), while others were Leishmania major (L. ). L. more variable compared with L. . The molecular sequencing differences between L. to geographical distribution. Based on the results of PCR product in the gel electrophoresis and DNA sequencing for L. L. , the DNA sizes were between 350 and 369bp. The RFLP for L. L. and one respectively. The sequences all samples from central parts are the same, but there is difference with the samples isolated from of Iran. The sequences of of Leishmania major separated from Damghan and Esfarayen are different from other samples. Similarity of DNA sequences of North-East part of Iran of L. from central parts was 99%. The similarity of two isolates of L. 96%. The most similarity of Leishmania isolated was 95% with Indian isolate and the most similarity for Leishmania major was 99% with Friedlin strain.