Molecular identification of Mutations causing resistance to tetracycline in mycoplasma isolated from patients with multiple sclerosis (Ms) in Kerman province
Message:
Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
Background
Bacterial infections on the initiation or escalation, play a role in MS, and reports of the involvement of ‎mycoplasma with worldwide distribution in these patients‏.‏‎ Mycoplasma with the host cell membrane ‎to mimic the autoimmune attack and the other macrolide antibiotics have become resistant to the first ‎treatment. The purpose of this research was to identify mutations causing resistance to tetracycline in ‎mycoplasmas isolated from patients with MS Kerman province.
Methods
A total of 32 samples of cerebrospinal fluid and 48 samples of urine results in a non-random sampling of ‎patients with MS prepared and enrich one night in PPLO broth, were gathered during a‎ 7 weeks of ‎continuous cultivation in PPLO broth and agar. DNA was extracted from positive examples to identify ‎the type of bacteria (Nested-PCR) and was used tetracycline resistant (rrs3 and rrs4) of 16SrRNA the ‎method (Duplex-PCR), as well as was performed nucleotide sequencing and phylogenetic tree.
Results
Growth was observed in 12 cases, but after (Nested-PCR), only 5 samples were detected (1 sample of ‎cerebrospinal fluid and urine samples 4) mycoplasma. But In (Duplex-PCR), to show only urine ‎samples, alleles‏.
Conclusion
The phylogenetic was found to have mutated alleles at different points, but for a definitive answer to ‎the resistance to tetracycline, additional research is required. Based on the findings, have mutated ‎alleles has the potential to tetracycline resistance.
Language:
Persian
Published:
Medical Journal of Tabriz University of Medical Science, Volume:41 Issue: 1, 2019
Pages:
100 to 107
magiran.com/p1954432  
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یک‌ساله به مبلغ 990,000ريال می‌توانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
  • حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران می‌شود.
  • پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانه‌های چاپی و دیجیتال را به کاربر نمی‌دهد.
دسترسی سراسری کاربران دانشگاه پیام نور!
اعضای هیئت علمی و دانشجویان دانشگاه پیام نور در سراسر کشور، در صورت ثبت نام با ایمیل دانشگاهی، تا پایان فروردین ماه 1403 به مقالات سایت دسترسی خواهند داشت!
In order to view content subscription is required

Personal subscription
Subscribe magiran.com for 50 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!