Effect of Salicylic Asid and Methyle Jasmonat Elicitors on Hypericin production in stJohn’s wort (Hypericum perforatum L.) cv. Topas Callus culture

Effects of salicylic acid and methyl Jasmonate on Hypericin production in callus culture of Hypericum perforatum L. cv Topas were investigated. This factorial experiment was carried out in a completely randomized design with two factors including the four levels of salicylic acid (225,150,75,0 µM) and methyl Jasmonate in three levels (150,75,0 µM) with three replications at the research lab Group of Sari University of Agricultural Sciences and natural resources, deprtment of Horticultural Science. Analysis of variance showed significant differences among treatments. Effect of treatments on total flavonoid Methyl Jasmonate effect was significant. Interaction effect was significant for Hypericin, flavonoids and anthocyanins. According to the results of this study in cell cultures of H. perforatum, low concentration (100 micromolar and lower) of both salicylic acid and methyl Jasmonate elicitors were effective for production of Hypericin. The results of this experiment showed that the highest amount of Hypericin produced from 150 μM salicylic acid (SA) and 225 μM salicylic acid in combination with 75 μM methyl Jasmonate (MJ) after 21 days.