Antimicrobial effect of Cardin (Biarum bovei) extract against Escherichia coli and Staphylococcus aureus: Studies in vitro and hamburger

Evaluation of the antimicrobial activity of plant extracts and essential oils has grown dramatically in recent years. In this study, the antimicrobial effect of Cardin extract (Biarum bovei) in vitro and hamburger on Escherichia coli and Staphylococcus aureus bacteria and total countt is investigated. At first, different concentrations of Cardin hydroalcholicextract was prepared. Antibacterial effects of extracts were evaluated by agar method (disc and wells), minimum inhibitory concentration and minimum bactericidal concentration (by dilution method) and time-killing curves. Antimicrobial activity of extract against Escherichia coli, Staphylococcus aureusand its effect on total microbial count in hamburger at -12 °C during storage (0, 15, 30 and 45 days) was studied. In diffusion method, with increasing concentration, the diameter of inhibition increased. MIC of extract for Escherichia coli and Staphylococcus aureus, was 25 and 12.5 mg/ml and MBC was 50 and 50 mg/ml, respectively. After treatment the hamburger samples with different concentrations of the extract, it was found that antimicrobial activity increased with increasing the concentration and decreasing the temperature. Thus, it can be concluded that hydroalcoholic Cardinextract can be replaced by synthetic materials as a natural antimicrobial compoundto improve durability.